Protease Enzyme from Trichoderma reesei
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Definitions

Trichoderma reesei
Trichoderma reesei is a mesophilic and filamentous fungus. Trichoderma reesei has the
capacity to secrete large amounts of cellulolytic enzymes.

Protease
A protease is any enzyme that conducts proteolysis - breaking down proteins.

Genencor
A company locates at Palo Alto, CA. Address: 925 Page Mill Road Palo Alto, CA 94304

Application of Trichoderma reesei
An acid fungal protease enzyme preparation from Trichoderma reesei expressing the
gene encoding acid fungal protease from T. reesei.

The Food and Drug Administration (FDA) is responding to the notice, dated March 29,
2010, that Genencor submitted in accordance with the agency’s proposed regulation,
proposed 21 CFR 170.36 (62 FR 18938; April 17, 1997; Substances Generally
Recognized as Safe (GRAS); the GRAS proposal). FDA received the notice on March
30, 2010, filed it on March 31, 2010, and designated it as GRAS Notice No. GRN 000333.

Commercial enzyme preparations that are used in food processing typically contain an
enzyme component, which catalyzes the chemical reaction that is responsible for its
technical effect, as well as substances used as stabilizers, preservatives, or diluents.
Enzyme preparations may also contain constituents derived from the production organism
and manufacturing process. In their notice, Genencor provides information about each of
these components of the AFP enzyme preparation.

SOURCE: FDA website 2010